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KMID : 0545119950050050297
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Journal of Microbiology and Biotechnology
1995 Volume.5 No. 5 p.297 ~ p.301
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Improved Production, and Purification of Aclacinomycin A from Streptomyces lavendofoliae DKRS
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Kim, Wan Seop
Youn, Deok Joong/Cho, Won Tae/Kim, Myung Kuk/Kim, Hak Ryul/Rhee Sang Ki/Choi, Eui Sung
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Abstract
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An anthracycline antibiotic, aclacinomycin A (aclarubicin), was produced from a mutant strain of Streptomyces lavendofoliae. The mutant strain which showed a 4-fold higher productivity of aclacinomycin A compared with the parent strain was also found to produce a significantly higher amount of aclacinomycin A than the reported production strain, Streptomyces galilaeus. The aclacinomycin A was produced up to 12.5 ㎎/ℓ using potato starch and soybean meal as carbon and nitrogen sources, respectively, on a 3 liter scale fermentation in a 5 liter jar fermentor. The mutant strain also produced significant amount of aclacinomycins B and Y. Aclacinomycin A was isolated from the culture broth by solvent extractions and further purified by silica gel column chromatography. The yield of aclacinomycin A with over 99% purity was found to be over 60% starting from 3 liters of culture broth.
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